Journal:
Article Title: Detection in Escherichia coli of the Genes Encoding the Major Virulence Factors, the Genes Defining the O157:H7 Serotype, and Components of the Type 2 Shiga Toxin Family by Multiplex PCR
doi: 10.1128/JCM.40.10.3613-3619.2002
Figure Lengend Snippet: Shown are multiplex-PCR amplification profiles from reference E. coli strains with primer set A (A), B (B), and C (C). (A) Lane 1, 100-bp DNA ladder (Bethesda Research Laboratories Inc., Gaithersburg, Md.); lane 2, stx1 and 16S rRNA (E. coli strain H19); lane 3, stx2 and 16S rRNA (strain 90-2380); lane 4, stx1, stx2, and 16S rRNA (strain 87-1215); lane 5, 16S rRNA (formerly stx2Va, strain 91-2245); lane 6, 16S rRNA (formerly stx2Vb, strain 86-704); lane 7, stx2 (stx2-stx2c,strain E32511); lane 8, stx1 and 16S rRNA (stx1-stx2d, strain 91-126); lane 9, 16S rRNA (stx2e, strain 412); lane 10, stx2f and 16S rRNA (stx2f, strain H.I.8); lane 11, stx1, stx2, stx2f, and 16S rRNA; lane 12, stx negative control (ATCC 25922). (B) Lane 1, 100-bp DNA ladder (Bethesda Research Laboratories Inc.); lane 2, amplification products of eaeA and 16S rRNA (stx1, strain H19); lane 3, eaeA and 16S rRNA(stx2, strain 90-2380); lane 4, eaeA and 16S rRNA(stx1-stx2, strain 87-1215); lane 5, eaeA, stx2c, and 16S rRNA (formerly stx2Va, strain 91-2245); lane 6, stx2c and 16S rRNA (formerly stx2Vb, strain 86-704); lane 7, eaeA, stx2c and 16S rRNA (stx2-stx2c, strain E32511); lane 8, 16S rRNA(stx1-stx2d, strain 91-126); lane 9, stx2e and 16S rRNA (stx2e, strain 412); lane 10, eaeA and 16S rRNA (stx2f, strain H.I.8); lane 11, eaeA, stx2c, stx2e, and 16S rRNA; lane 12, stx negative control (ATCC 25922). (C) Lanes 1 and 12, 100-bp DNA ladder (Bethesda Research Laboratories Inc.); lane 2, hlyA and 16S rRNA (strain H19); lane 3, hlyA, rfbEO157, fliCH7, and 16S rRNA(strain 90-2380, O157:H7); lane 4, hlyA, rfbEO157, fliCH7, and 16S rRNA (strain E32511, O157: NM); lane 5, rfbEO157 and 16S rRNA (O157: H21); lane 6, fliCH7 and 16S rRNA (O55: H7); lane 7, hlyA, stx2d, and 16S rRNA (stx1-stx2d, strain 91-126); lane 8, 16S rRNA (strain 412); lane 9, 16S rRNA (strain H.I.8); lane 10, positive control of hlyA, rfbEO157, fliCH7, stx2d, and 16S rRNA; lane 11, stx negative control (ATCC 25922).
Article Snippet: The control strains had been previously defined in terms of virulence factors and toxigenicity with respect to the genes stx 1 , s tx 2 , s tx 2c , stx 2 - stx 2c , stx 2d , stx 2e , stx 2f , EHEC hlyA , and eaeA (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Serotype Stx gene(s) a EHEC genotype Reference(s) or source H19 O26:H11 stx 1 ( SLT-I ; VT1) 3 933W O157:H7 stx 2 ( SLT-II ; VT2) 16 87-1215 O157:H7 stx 1 , stx 2 This study B2F1 O91:H21 stx 2c ( SLT-IIvha , SLT-IIvhb ; VT2c) 15 86-704 O15:H27 stx 2c ( SLT-IIvhb ; VT2c) This study {"type":"entrez-nucleotide","attrs":{"text":"E32511","term_id":"13026758","term_text":"E32511"}} E32511 O157:NM stx 2 , stx 2c ( SLT-II , SLT-IIc ) 32 91-126 O128:H? stx 1 + stx 2d (Stx1 + Stx2d) 31 ; this study 412 O139:K82 stx 2e ( SLT-IIe ; VTe) 11 , 40 H.I.8 O128:B12 stx 2f ( SLT-IIva ; VTeV) 7 90-2380 O157:H7 stx 2 hlyA eaeA This study 25922 O6:H1 Lacking American Type Culture Collection 92-3136 O157:H21 Lacking This study 91-0575 O55:H7 Lacking This study Open in a separate window a Data in parentheses are Shiga-like toxin genes, Stx toxins, and/or verotoxins.
Techniques: Multiplex Assay, Amplification, Negative Control, Positive Control
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